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In this study, we use comparisons between the gene expression profiles of wild-type C. diphtheriae strain 1737 grown without or with Mn supplementation and comparisons of gene expression between the wild type and an deletion mutant to characterize the C. diphtheriae Mn and MntR regulon. MntR was observed to both …
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Daha fazla öğreninEurope PMC is an archive of life sciences journal literature.
Daha fazla öğreninThus, the crack resistance of MNTR/SBS hybrid modified asphalt binder at low service temperature had potential for being improved. Fig. 6 c shows SEM image of MNTR, and core–shell structured carbon black was observed on nanoscale level. As a common reinforcing agent, carbon black in MNTR strongly contributed to the strength …
Daha fazla öğreninBacterial strains and media. The wild-type C. diphtheriae strain C7(−) has been described ().Strain 1716 is a clinical isolate from the recent diphtheria epidemic in the former Soviet Union and was provided by Tanya Popovich (Centers for Disease Control and Prevention, Atlanta, Ga.).C7(β)hm723 is derived from the C7 strain and carries a point mutation in …
Daha fazla öğreninThe Bacillus subtilis MntR and Zur transcriptional regulators control homeostasis of manganese and zinc, two essential elements required in various cellular …
Daha fazla öğreninWe also note that in an mntR null mutant background, in which Mn(II) importers are expressed constitutively, mutation of either mneP or mneS confers increased Mn(II) sensitivity, and the triple mutant (mntR mneP mneS) displays exceptionally high Mn(II) sensitivity (Fig. 2A), which can be complemented by the ectopic expression of …
Daha fazla öğreninThe manganese transport regulator (MntR) of Bacillus subtilis is a metalloregulatory protein responsible for regulation of genes involved in manganese uptake by this organism. MntR belongs to the iron-responsive DtxR family, but is allosterically regulated by manganese and cadmium ions. Having previously characterized the metal …
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Daha fazla öğreninThe mntR mutant SIP933 was complemented with pSP116/1 (plasmid carrying mntR), and the fur mutant SIP890 was complemented with pSP61/18 (plasmid carrying fur). Cells were grown aerobically for 6 h in MA medium (white bars) or in MA medium amended with 5 μM (black bars) or 20 μM (gray bars) metal ions, and β-galactosidase activities were then ...
Daha fazla öğreninManganese transport regulator (MntR) is a member of the diphtheria toxin repressor (DtxR) family of transcription factors that is responsible for manganese homeostasis in Bacillus subtilis. Prior biophysical studies have focused on the metal-mediated DNA binding of MntR [Lieser, S. A., Davis, T. C., …
Daha fazla öğreninsubroutine mntr_logrv(mid, priority, logs, rvarv, rvarn) Write log message adding real vector of length n. Definition: mntrlog.f:755. mntr_mod_get_olist. subroutine mntr_mod_get_olist(olist, ierr) Provide ordered list of registered modules for . Definition: mntrlog.f:929.
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Daha fazla öğrenin2 PRELUCRAREA PRIN FREZARE 2.1. Scopul lucrării Cunoaşterea echipamentului tehnologic şi a posibilităţilor de prelucrare prin frezare. 2.2. Definirea procedeului şi schema de principiu Frezarea este procedeul de prelucrare prin aşchiere rezultat din combinarea unei mişcării principale de aşchiere, totdeauna de rotaţie şi totdeauna ...
Daha fazla öğreninThe Bacillus subtilisMntR and Zur transcriptional regulators control homeostasis of manganese and zinc, two essential elements required in various cellular …
Daha fazla öğreninEscherichia coli uses manganese [Mn(II)] as an essential trace element; thus, it has a genetic system that regulates cellular Mn(II) levels. Several genes in the mnt-operon of E. coli respond to intercellular Mn(II) levels, and transcription is regulated by a transcription factor (MntR) that interacts with Mn(II). This study aimed to develop Mn(II) …
Daha fazla öğreninThe Bacillus subtilis MntR and Zur transcriptional regulators control homeostasis of manganese and zinc, two essential elements required in various cellular processes. In this work, we describe the global impact of mntR and zur deletions at the protein level. Using a comprehensive proteomic approach, we showed that 33 and 55 …
Daha fazla öğreninThe Bacillus subtilis manganese transport regulator, MntR, binds Mn2+ as an effector and is a repressor of transporters that import manganese. A member of the diphtheria toxin repressor (DtxR ...
Daha fazla öğreninAbstract. The Bacillus subtilis manganese transport regulator, MntR, binds Mn 2+ as an effector and is a repressor of transporters that import manganese. A member of the diphtheria toxin...
Daha fazla öğreninBFGCL0813I: A request to stop resource monitor 'MNTR' of agent 'SOURCE' has been issued. BFGCL0815I: Resource monitor 'MNTR' of agent 'SOURCE' is already stopped. 5) Submit a command to start a resource monitor in an agent tha t is stopped C:UsersAdministrator>fteStartMonitor -mn MNTR -ma SOURCE
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Daha fazla öğreninMntR of Bacillus subtilis was the first manganese-responsive DtxR-type regulator that has been characterized. It was shown to repress the two manganese …
Daha fazla öğreninMntR is a direct transcription activator of the mneP and mneS genes. Multiple round in vitro transcriptions were conducted for (A) Mn (II) uptake genes mntH and mntA and (B) Mn …
Daha fazla öğreninTo identify genes positively regulated by MntR, we performed the microarray analysis with RNA extracted from wild-type and ΔmntR cells exposed to 10 μM MnCl for 10 min (GEO …
Daha fazla öğreninMntR belongs to the DtxR/MntR family of metallosensors, which consists of Fe 2+-sensing transcription factors such as DtxR and IdeR, and of Mn 2+-sensing transcription factors such as SloR, ScaR …
Daha fazla öğreninBiP and fused to MntR using synthetic PCR with long oli-gonucleotides of the targeting sequences. A Golgi targeted version of MntR was generated by fusion to the full length Golgi native protein Cab45. The whole mCherry-Cab45 gene was synthesized and included convenient sites for fusion of the Cab45 element into MntR (Cab45MntR) or
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Daha fazla öğreninManganese is a micronutrient required for activities of several important enzymes under conditions of oxidative stress and iron starvation. In Escherichia coli, the manganese homeostasis network primarily constitutes a manganese importer (MntH) and an exporter (MntP), which are regulated by the MntR dual regulator.In this study, we find that …
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Daha fazla öğreninLane 1, free DNA; lanes 2 through 6 contain 0.5 mM Mn (II) plus MntR at a final concentration of 2 µM (lane 2), 400 nM (lane 3), 80 nM (lane 4), 16 nM (lane 5) or 3.2 nM (lane 6). In this experiment, a concentration of MntR of ∼ 80 nM leads to half-maximal shifting of the labelled DNA fragment.
Daha fazla öğrenin33 Recent experiments reveal that the A-site Mn(II) ion in the A/C cluster can be replaced by a positively charged Lys ε-NH 2 chain via mutagenesis (E11K) in MntR with the retention of full ...
Daha fazla öğreninThe pathogenic mycobacterium Mycobacterium tuberculosis encodes two members of the DtxR/MntR family of metalloregulators, IdeR and SirR. IdeR represses gene expression in response to ferrous iron, and we here demonstrate that SirR (Rv2788), although also annotated as an iron-dependent repressor, functions instead as a …
Daha fazla öğreninManganese transport regulator (MntR) is a member of the diphtheria toxin repressor (DtxR) family of transcription factors that is responsible for manganese homeostasis in Bacillus subtilis.Prior biophysical studies have focused on the metal-mediated DNA binding of MntR [Lieser, S. A., Davis, T. C., Helmann, J. D., and Cohen, …
Daha fazla öğreninThe bacterial Mn-binding protein, MntR was identified as a candidate reporter protein. MntR was engineered for expression in mammalian cells, and targeted to different subcellular organelles, including the Golgi Apparatus where cellular Mn is enriched. Transfected HEK293 cells and B16 melanoma cells were tested in vitro and in vivo, using ...
Daha fazla öğreninThe manganese transport regulator (MntR) of Bacillus subtilis is a metalloregulatory protein responsible for regulation of genes involved in manganese uptake by this organism. …
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